Installing Stage Inserts; Aperture Diaphragm - Optika Italy Im Serie Manual De Instrucciones

10.8.1 Installing stage inserts

1. Install the holder in the mechanical stage. (Fig. 19)
2. Multi well plates can be directly inserted in the mechanical
stage. (Fig. 20)

10.9 Aperture diaphragm

The Numerical Aperture (N.A.) value of the aperture diaphragm
affects the image contrast. Increasing or reducing this value one
can vary resolution, contrast and depth of focus of the image.
With low contrast specimens move the Aperture Diaphragm lever
(AS) ① to set the numerical aperture to about 70%-80% of the
objective's N.A. (Fig. 21)
If necessary, remove one eyepiece and, looking into empty
sleeve, adjust the aperture diaphragm ring in order to obtain an
image like the one in Fig. 22.
APERTURE
DIAPHRAGM
FIELD OF VIEW
Page 15
F F ig. 19 ig. 19
F ig. 20 F ig. 20
①
F ig. 21 F ig. 21
70-80%
30-20%
F ig. 22 F ig. 22